电感耦合等离子体原子发射光谱法测定L-肌肽中铁和铬的含量

ICP-AES Determination of Fe and Cr in L-Carnosine

摘要: 采用电感耦合等离子体原子发射光谱法测定L-肌肽中铁和铬的含量。选择高温干法灰化法对L-肌肽样品进行前处理。ICP-AES测试条件如下：每种元素各选择两条谱线,铁的分析谱线为238.204 nm和259.939 nm,铬的分析谱线为267.716 nm和283.563 nm;发射功率为1 300 W;雾化气流量为0.8 L·min-1。铁和铬的质量浓度在一定范围内与其发射强度呈线性关系,方法的检出限(3s/k)在0.002~0.012 mg·L-1之间。加标回收率在92.0%~104%之间,测定值的相对标准偏差(n=6)小于8.0%。

Abstract: ICP-AES was applied to the determination of Fe and Cr in L-carnosine. The sample was pretreated by high temperature dry ashing method. The test conditions of ICP-AES were as follows: analytical spectral lines of 238.204, 259.939 nm for the determination of Fe and 267.716, 283.563 nm for Cr; emission power of 1 300 W; nebulizer gas flow rate of 0.8 L·min-1. Linear relationships were found between emission intensity and the mass concentration of Fe, Cr in definite ranges with detection limits (3s/k) in the range of 0.002-0.012 mg·L-1. Recovery rates obtained by standard addition method were in the range of 92.0%-104%, and RSDs (n=6) were less than 8.0%.